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International Journal of Advance Scientific Research SCIENTIFIC IDEAS. INTERNATIONAL PERSPECTIVES.
ISSN 2750-1396 12 issues per year · Monthly
Crossref DOI: 10.37547/ijasr
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Articles Published:

POLYMORPHIC INSIGHTS: DEVELOPMENT OF SIMPLE SEQUENCE REPEAT MARKERS FOR STUDYING DNA POLYMORPHISMS IN SORGHUM AND MILLET CULTIVARS

Vol. 3 No. 06 (2023): Volume 03 Issue 06 Pages 183-186
Authors
  • Roja Navishta
    Department of Genetics and Biotechnology, University College for Women, Koti, Hyderabad, Telangana, India
RESEARCH OVERVIEW

Abstract

Understanding DNA polymorphisms is essential for studying the genetic diversity and population structure of crop plants. This study aimed to develop simple sequence repeat (SSR) markers for investigating DNA polymorphisms in sorghum and millet cultivars. SSR markers, known for their high information content, co-dominant inheritance, and reproducibility, were designed and synthesized based on the reference genome sequences of sorghum and millet. These SSR markers provide valuable tools for further genetic analysis and breeding programs in sorghum and millet cultivars.

Keywords

  • DNA polymorphisms
  • simple sequence repeat markers
  • sorghum
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References

Gupta, P.K., Rustgi, S., Sharma, S., Singh, R., Kumar, N., Balyan, H.S. (2003). Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Molecular Genetics and Genomics, 270(4), 315-323.
Varshney, R.K., Graner, A., Sorrells, M.E. (2005). Genic microsatellite markers in plants: features and applications. Trends in Biotechnology, 23(1), 48-55.
Billot, C., Ramu, P., Bouchet, S., Chantereau, J., Deu, M., Gardes, L., ... & Courtois, B. (2013). Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources. PLoS ONE, 8(4), e59714.
Rajaram, V., Nepolean, T., Senthilvel, S., Varshney, R.K., Vadez, V., Srivastava, R.K., ... & Gupta, R.K. (2013). Pearl millet [Pennisetum glaucum (L.) R. Br.] consensus linkage map constructed using four RIL mapping populations and newly developed EST-SSRs. BMC Genomics, 14(1), 159.
Ramu, P., Billot, C., Rami, J.F., Senthilvel, S., Upadhyaya, H.D., Reddy, L.A., ... & Hash, C.T. (2013). Assessment of genetic diversity in the sorghum reference set using EST-SSR markers. Theoretical and Applied Genetics, 126(8), 2051-2064.
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